Figure 5. Disruptions of cell-matrix and cell-cell interactions by F14 and EGTA abolish the protective effects of ROCK1 deletion in response to doxorubicin. MTT assay performed with WT and ROCK1^−/− MEFs with 1 μM doxorubicin and/or 1 μM F14 (A) or 2 mM EGTA (B) for 16 h. Cell viability was expressed as percentage of control cells without treatment. *p < 0.05 vs. control of the same genotype; ^#p < 0.05 vs. WT under the same treatment condition. ^¶p < 0.05 vs. the same genotype under doxorubicin only condition.