Figure 7. ROCK1 deletion inhibits apoptosis induced by serum starvation in attached cells. (A) Representative image (left panel) of western blot of full-length and cleaved ROCK1 and cleaved caspase-3 in cell lysates from attached WT and ROCK1^−/− MEFs before and after 4 or 8 h of serum starvation. Quantitative analysis (right panel) of immunoreactive bands of cleaved capsase-3 (n = 4–6 for each condition), expressed as percent change relative to WT cells after 8 h of serum starvation. *p < 0.05 vs. control of the same genotype; ^#p < 0.05 vs. WT under the same treatment condition. (B) Representative image (left panel) of western blot of full-length and cleaved PARP, cleaved caspase-3 in cell lysates from attached or floating WT cells after 4 or 8 h of serum starvation. Densitometry analysis (right panel) of immunoreactive bands of cleaved caspase-3. Expression of cleaved caspase-3 was expressed as fold change relative to attached cells after 8 h of serum starvation. *p < 0.05 vs. 8 h attached cells.