Figure 2. p53 and miR-34 inversely correlated with Axin2 expression. (A) Endogenous Axin2 level was determined by immunoblot analysis in a panel of colorectal cancer cell lines according to the p53 functional status. Tubulin was used as the loading control. (B) The transcript levels of Axin2 and pri-miR-34a (upper panel) and mature miR-34a (lower panel) were determined by quantitative RT-PCR analysis in a panel of colorectal cancer cells. Results are expressed as the mean ± SD of triplicate experiment. (C) Immunoblot analysis of endogenous p53 and Axin2 protein levels after treatment of 0.1 mg/ml adriamycin (ADR) for 8 h in colorectal cancer cells. (D) Quantitative RT-PCR analysis of pri-miR-34a transcript levels after treatment of ADR. Results are expressed as the mean ± SD of triplicate experiment.