Figure 3. Genetic interactions between elg1Δ, chl1Δ and mhf1Δ and mhf2Δ mutants. (A) Tetrad analysis of a cross between a elg1Δ and a chl1Δ strain showing a synthetic fitness phenotype for the elg1Δ chl1Δ double mutant. Generation times were measured for six independent spores of each phenotype. (B) Tetrad analysis show no synthetic fitness interactions between chl1Δ and mutations that abolish the PCNA interacting motif (PIP), the SUMO interacting motif of Elg1 or both. (C) Drop test on MMS. (D) Drop test on HU.