Fig. 2.
Wild-type and mutant connexins are equivalently expressed in Xenopus oocytes. A: equal amounts of membrane extracts were first probed with an antibody that recognized Cx26. H2O-injected controls did not express Cx26 as expected. Wild-type Cx26, Cx26-D50A, and Cx26-A88V were readily detected in lanes corresponding to each injection condition with similar band intensities. B: to confirm equal sample loading, blots were stripped and reprobed with an antibody against β-actin, which was present at comparable levels in all lanes.