Fig. 7.

Expression of Cx26-D50A and Cx26-A88V in transfected human keratinocytes. Untransfected primary human keratinocytes (A, blue DAPI stain) displayed a strong endogenous wild-type Cx26 (red) labeling that concentrated at cell-to-cell interfaces, which was most clearly seen in isolated pairs of cells (arrowhead in inset). Keratinocytes transfected with either Cx26-D50A (B) or Cx26-A88V (C) displayed an increased Cx26 labeling that correlated with GFP expression (green), although the Cx26 signal was no longer focused at cellular appositions. Staining of cell borders with rhodamine-conjugated wheat germ agglutinin (D-F), revealed that the expression of Cx26-D50A (E) or Cx26-A88V (F) did not markedly alter cell morphology.