Fig. 5.
Pemetrexed preservation of transport function after modification of PCFT mutants by MTSET. Transient transfectants of G147C, A152C, F157C, G158C, and L161C mutants were divided into 3 groups for MTSET (3 mM) treatment at 0°C for 30 min: incubation in HBS (no treatment), MTSET, and MTSET + 1 mM pemetrexed. After MTSET treatment, cells from all groups were washed 3 times with HBS to remove MTSET and pemetrexed before [3H]MTX (0.5 μM) influx was measured at pH 5.5 and 37°C over 1 min. Values are means ± SE from 4 independent experiments. *P < 0.05 vs. MTSET. Differences in influx between cells treated with MTSET + pemetrexed and cells not treated with MTSET (no treatment) are not statistically significant (P > 0.05).