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. 2013 Mar 13;304(11):F1375–F1389. doi: 10.1152/ajprenal.00020.2013

Fig. 4.

Fig. 4.

Prednisone decreases podocyte apoptosis in experimental FSGS. A–C: podocyte apoptosis was measured by double staining for caspase-3 (red color) and synaptopodin (green color), with DAPI staining nuclei (blue color; ×630 original magnification). A1–3: normal mice: synaptopodin staining is present in a typical podocyte distribution (arrows); caspase-3 staining is absent. B1–3: mice with FSGS given DMSO. B-1: caspase-3-stained cell (arrowhead) is shown. B-2: areas of reduced synaptopodin staining are noted (*). B-3: caspase-3 and synaptopodin double positive cell (yellow color, arrowhead) in the tuft. C1–3: mice with FSGS given prednisone. C-1: caspase-3-positive cells in the tubule (dashed arrow) adjacent to the glomerulus. C-2: intensity of synaptopodin staining is more marked in mice with FSGS given DMSO. C-3: no double staining is detected in the glomerulus. D: quantitation for caspase-3+/synaptopodin+ double staining: apoptosis is increased in mice with FSGS given DMSO at 2 and 4 wk (hatched bars) compared with normal mice (open bars). Prednisone treatment decreased apoptosis at 2 and 4 wk (filled bars).