Skip to main content
. 2013 Mar 13;304(11):F1375–F1389. doi: 10.1152/ajprenal.00020.2013

Fig. 7.

Fig. 7.

Glomerular progenitor cells, defined as cells double staining for PAX2 (blue/gray) and Wilms' Tumor-1 (WT-1; red), increase in experimental FSGS. A: representative images of PAX2+/WT-1+ double staining at ×630 original magnification in normal mice. A-1: arrows indicate PAX2 (blue/gray)/WT-1 (red) double staining at 2 wk. Arrows indicate examples of a PAX2-positive (blue/gray only, WT-1-negative) cell and a WT-1-positive (red only, PAX2-negative) cell. A-2: fluorescent microscopic view of the A-1 bright-field view, where only WT-1 staining is seen because only the warp-red substrate is visible by fluorescent microscopy. * Indicates the site where PAX2 stains but WT-1 (red fluorescence) is negative in the nucleus. A-3 and A-4: represent PAX2/+WT-1+ staining in normal mice at 4 wk. Arrows indicate PAX2 or WT-1 single positive cells, and * indicates the site where PAX2 stains but WT-1 is negative in the nucleus (A-4). B: representative images of PAX2+/WT-1+ double staining at ×630 original magnification in untreated mice with FSGS at 2 wk. B-1: arrows indicate PAX2 (blue/gray) or WT-1 (red) single positive cells. Arrowhead indicates a PAX2+/WT-1+ double positive cell lining along Bowman's capsule. B-2: fluorescent microscopic view of B-1 bright-field view, where only WT-1 staining is seen. Arrowhead indicates the same cell from B-1, termed glomerular progenitor cell, which is visible. B-3 and B-4: same staining in untreated mice with FSGS at 4 wk. Arrows indicate PAX2 or WT-1 single positive cells, arrowheads indicate a glomerular progenitor cell (PAX2+/WT-1+ double positive). C: representative images of PAX2+/WT-1+ double staining at ×630 original magnification in prednisone-treated mice with FSGS at 2 wk. C-1: arrows indicate PAX2 (blue/gray) or WT-1 (red) single positive cells. Arrowheads indicate PAX2+/WT-1+ double positive cells lining Bowman's capsule and in the glomerular tuft. C-2: fluorescent microscopic view of the C-1 bright-field view, only WT-1 staining is seen. Arrowheads indicate the same cells from C-1, which are visible by fluorescence. C-3 and C-4: same staining in prednisone-treated mice with FSGS at 4 wk. Arrows indicate PAX2 or WT-1 single positive cells; arrowheads indicate glomerular progenitor cells (PAX2+/WT-1+ double positive). D: number of cells staining positive for PAX2 and WT-1 per glomerular cross section was significantly higher at 2 and 4 wk in untreated mice with FSGS (hatched bar) compared with normal mice (open bar). Prednisone treatment increases the number of PAX2/WT-1-positive cells/glomerular cross section at 2 and 4 wk (filled bars), compared with untreated mice with FSGS.