Fig. 6.

ZSCAN4 expression in pancreatic cells in adult human pancreas. A: colocalization of LGR5 and ZSCAN4 in the islet of Langerhans. LGR5⁺ cells are located at the peripheral region of the islet. Only a small portion of LGR5⁺ cells are positive for ZSCAN4 in the islet of Langerhans. White dashed line indicates the margin of the islet. B: LGR5 and BMI1 expression in the islet of Langerhans. Some of the LGR5⁺ cells are also positive for BMI1. C: ZSCAN4 is expressed in a mature AQP1⁺ cell apart from pancreatic ducts. Note that ZSCAN4⁺ cells are clearly identifiable in a DIC image. D: AQP1, amylase, and insulin (INS) triple-positive intermediate mixed exocrine and endocrine cells located in the interstitium of the human pancreas. Note that these cells are clearly identifiable in a DIC image. E: an insulin-positive cell in pancreatic acini. F: amylase and insulin double-positive oval-shaped cell is also positive for the undifferentiated endocrine cell marker NEUROG3. G: a BMI1 and AQP1 double-positive cell at the interstitium of the human pancreas. H: AQP1 and insulin double-positive oval cells also express SSEA3. Bars, 50 μm.