Table 1.
List of antibodies used in this study
| Antibodies | Species | Manufacturer | Product ID | Working Dilution |
|
|---|---|---|---|---|---|
| Anti-ALDH | Rabbit | Abcam | ab52492 | IHC | 1:100 |
| IF | 1:100 | ||||
| Anti-AQP1 | Rabbit | Alpha Diagnostic | AQP11-A | IHC | 1:500 |
| IF | 1:500 | ||||
| Anti-α-amylase | Mouse | Abcam | ab54765 | IHC | 1:300 |
| IF | 1:100 | ||||
| Anti-αSMA | Mouse | Abcam | ab7817 | IHC | 1:100 |
| IF | 1:100 | ||||
| Anti-BMI1 | Mouse | Millipore | 05-637 | IHC | 1:200 |
| IF | 1:100 | ||||
| Anti-CAII | Rabbit | Abcam | ab6621 | IHC | 1:2,000 |
| IF | 1:1,000 | ||||
| Anti-CD163 | Mouse | Leica | NCL-CD163 | IHC | 1:300 |
| IF | 1:100 | ||||
| Anti-DCAMKL-1 | Rabbit | Abcam | ab37994 | IHC | 1:100 |
| IF | 1:100 | ||||
| Anti-desmin | Rabbit | Abcam | ab32362 | IHC | 1:500 |
| IF | 1:200 | ||||
| Anti-GFAP | Rabbit | Abcam | ab16997 | IHC | 1:100 |
| IF | 1:100 | ||||
| Anti-glucagon | Rabbit | Abcam | ab18461 | IHC | 1:5,000 |
| IF | 1:1,000 | ||||
| Mouse | Abcam | ab10988 | IHC | 1:4,000 | |
| IF | 1:1,000 | ||||
| Anti-insulin | Guinea pig | Abcam | ab7842 | IHC | 1:500 |
| IF | 1:2,000 | ||||
| Mouse | Abcam | ab7760 | IHC | 1:3,000 | |
| IF | 1:2,000 | ||||
| Anti-LGR5 | Rabbit | Abcam | ab75732 | IHC | 1:300 |
| IF | 1:100 | ||||
| Anti-NEUROG3 | Rabbit | Abcam | ab38548 | IHC | 1:100 |
| IF | 1:100 | ||||
| Anti-SSEA3 | Rat | Abcam | ab16286 | IHC | 1:100 |
| IF | 1:100 | ||||
| Anti-mouse Zscan4 | Rabbit | NIH | IHC | 1:2,000 | |
| IF | 1:500 | ||||
| Anti-human ZSCAN4 | Mouse | Abnova | H00201516-B01P | IHC | 1:200 |
| IF | 1:100 | ||||
| Alexa Fluor 488 anti-mouse | Goat | Invitrogen | A11017 | IF | 1:500 |
| Alexa Fluor 488 anti-rabbit | Goat | Invitrogen | A11070 | IF | 1:500 |
| Alexa Fluor 488 anti-rat | Chicken | Invitrogen | A21470 | IF | 1:500 |
| Alexa Fluor 594 anti-mouse | Goat | Invitrogen | A11020 | IF | 1:500 |
| Alexa Fluor 594 anti-rabbit | Goat | Invitrogen | A11072 | IF | 1:500 |
| Alexa Fluor 594 anti-guinea pig | Goat | Invitrogen | A11076 | IF | 1:500 |
ALDH, aldehyde dehydrogenase 1; AQP1, aquaporin 1; αSMA, α-smooth mucle actin; BMI1, B lymphoma Mo-MLV insertion region 1 homolog; CAII, carbonic anhydrase II; DCAMKL-1, doublecortin and CaM kinase-like-1; GFAP, glial fibrillary acidic protein; LGR5, leucine-rich repeat-containing, G protein-coupled receptor 5; NEUROG3, neurogenin 3; SSEA3, stage-specific embryonic-antigen 3; ZSCAN4, zinc finger and SCAN domain containing 4; IHC, immunohistochemistry; IF, immunofluorescent.