Table 7. Oligonucleotides used for cloning and BAC engineering.

No	Cloning/BAC engineering purpose	Primer sequence (5′→3′)
1a	HOT1 BAC stitching primers to add missing CDS and 3′-UTR, forward primer	CGATTTACCTGGAGAAAGGAGTGCCTGGCTGTTATGGAAAGT-TACTTCAATGAGAATCAATACCCAGATG
1b	HOT1 BAC stitching primers to add missing CDS and 3′-UTR, reverse primer	TATAATACAGCATTTATGATATTCTAAAGTACTTTTAGAGAT-AGAACCAACCCTGTGCTGCTACATTGAA