Figure 4.

T. gondii myosin F is associated with the apicoplast during parasite division. (A) TgMyoF (DQ131541) contains six IQ motifs, a coiled-coil domain and seven WD40 domains. All domains were predicted using SMART EMBL. (B) TgMyoF-3Ty is found at the expected molecular mass by western blot (216 kDa). Detection of T. gondii catalase was used as loading control. (C) MyoF-3Ty concentrates at the extremities of the apicoplast during division (arrow, upper panel) and to the newly formed daughter cells after apicoplast fission (arrowhead, upper and middle panels). In nondividing parasites (lower panel), TgMyoF is concentrated in the apical region, on top of the nucleus and at the pellicle. Scale bar, 2 μm.