Fig. 8.

Y300F mutated Hsp90β expression resists LPS-induced endothelial barrier dysfunction: PBS (vehicle) or LPS was added to the media of confluent transfected HLMVEC monolayers at 0 h. A gradual increase in endothelial permeability (reduced TER) was observed in the LPS-treated cells. The myc-Y300F-Hsp90β-transfected cells resisted to the LPS-induced endothelial barrier dysfunction: *,#P < 0.001 vs. corresponding untreated group of transfected cells; n = 4 per group. Error bars represent SE (A). Western blot analysis of the expression levels of Hsp90β and Hsp90 in HLMVEC transfected with myc-Y300F-Hsp90β and myc-wt-Hsp90β. The β-actin band was used as a loading control (B).