Fig. 2.

Upregulation of calcitonin-receptor-like receptor (CRLR) in differentiated myofibroblasts and in the lungs from bleomycin treated mice. A and B: time-dependent effect of TGF-β1 (1 ng/ml, 72 h) on the expression of CRLR mRNA (A) and on VASP phosphporylation (B) in human lung fibroblasts. C and D: effect of SB431542 (10 μM) on TGF-β1 (1 ng/ml, 3 days)-induced CRLR mRNA expression (C) or on VASP phosphorylation induced by 100 nM ADM or 1 μM iloprost (D). The % P-VASP is indicated below the VASP Western blots (B and D). E and F: bleomycin-induced upregulation of CRLR in mouse lungs. Pulmonary fibrosis was induced in 8- to 10-wk-old male C57Bl/6 mice by a single intratracheal administration of 1 U/kg bleomycin for 21 days. Lungs were then collected and analyzed for CRLR mRNA by real-time qPCR (E), or paraffin-embedded sections were stained for H&E (F). Shown are means ± SD from five lungs per condition (E).