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Recombinant ADM does not inhibit initial differentiation of quiescent fibroblasts. Quiescent human lung fibroblasts were pretreated with 100 nM ADM for 5 min followed by stimulation with 1 ng/ml TGF-β1 for 24 h. Cell extracts were then analyzed for the expression of desired mRNAs by real-time qPCR (A) or for proteins by Western blotting (B). The representative densitometry expressed as percent of TGF-β-treated condition is indicated below the corresponding Western blots.
