Fig. 6.

Bleomycin-induced pulmonary fibrosis is decreased in mice with transgenic overexpression of RAMP2. Pulmonary fibrosis was induced in 8- to 10-wk-old male wild-type (WT) C57Bl/6 mice or mice with transgenic overexpression of RAMP2 under the control of SM-α-actin promoter (RAMP2-tg) by a single intratracheal administration of 1 U/kg bleomycin for 15 days. A: survival curves for WT and RAMP2-tg mice. B: lung sections were stained for H&E, and scored blindly (using the Ashcroft scoring system) over 20–25 fields (×10 magnification) covering the whole lung section, and the average score and standard deviation were calculated and plotted. C: collagen content in mouse lungs as measured by hydroxyproline assay. D: representative images (×20 magnification) of lung sections from bleomycin-treated WT or RAMP2-tg mice, stained with H&E or SM-α-actin antibodies.