Fig. 6.

RelB overexpression increases IL-1β-induced miRNA-146a (miR-146a) production in human lung fibroblasts. Normal adult human lung fibroblasts were transfected with either a GFP control plasmid or a RelB-GFP expression plasmid, serum starved for 24 h, and treated with IL-1β at 1 ng/ml for 8 h. Total RNA was extracted, and miRNA production was detected by quantitative RT-PCR. Production of miR-146a was significantly increased upon IL-1β treatment, and IL-1β-induced miRNA-146a production was significantly increased upon RelB downregulation (D). miR-21, miR-101, miR-125a, and miR-155 were not significantly affected by either IL-1β or RelB overexpression in our model (A–C and E). *P < 0.05 and ***P < 0.001 by two-way ANOVA with a Bonferroni post hoc test (n = 3/group).