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eB shift competition with specific 14-3-3 antibody (Ab). REMSA of eB biotinylated probe with NCI-H441 cytoplasmic extract in the presence of Ab against all isoforms of the 14-3-3 protein family. Lanes 1 and 7, eB probe (negative controls); lanes 2 and 6, eB riboprobe incubated with extract (positive controls); lanes 3 and 4, 14-3-3 Ab disrupts the eB-specific shifts; lane 5, eB riboprobe incubated with extract and an irrelevant control anti-DDK Ab. S, specific shift.
