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. 2013 Apr 12;304(12):H1680–H1696. doi: 10.1152/ajpheart.00070.2013

Fig. 1.

Fig. 1.

Changes in intracellular pH (pHi) and intracellular H+ concentration ([H+]i) induced by extra- and intracellular acidosis and by pipette acid loading. A: a reduction in extracellular pH (pHo) from 7.4 to 6.5 for 2 min elicited only a small drop in pHi. B: in contrast, exposure to 80.0 mM acetate (pHo 7.4) induced a rapid sustained fall in pHi. C: example record showing the marked difference between intracellular acidosis (pHo 7.4) induced with 80 mM acetate and with pipette dialysis [pipette pH (pHp) = 6.6]. D: summarized results for extracellular acidosis (n = 7), intracellular acidosis (n = 17), and pipette acid loading (n = 4). We previously reported the summarized results shown in D for extra- and intracellular acidosis (41). The example records in A and B have not been previously published. All results shown here were obtained from a mixture of cells from all regions of the left ventricle. **P < 0.01 paired, control vs. acidosis. In separate experiments, we found no significant difference between the drop in pHi induced by 80.0 mM acetate (pHo 7.4) in epicardial (Epi, n = 4) and papillary muscle (PM, n = 7) myocytes (data not shown).