Fig. 9.
Effects of intracellular acidosis on human (h)Kv4.3 and hKv1.4 channels expressed in HEK 293T cells. A: example of pHi measurement in a nontransfected HEK 293T cell showing the effects of a 1-min exposure to 40.0 mM acetate (pHo 7.4). B, a: low pHi depressed the I-V curve for hKv4.3 at nearly all voltages (n = 4). Inset: example experiment showing the inhibitory effect of low pHi on hKv4.3 current elicited at +60 mV. b: In contrast, the I-V curve for hKv1.4 current was unaffected by low pHi (n = 4). Inset: example experiment showing the lack of effect of low pHi on peak hKv1.4 current elicited at +60 mV. *P < 0.05, paired, control vs. acidosis.