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ScPif1 preferentially binds G4 DNA. (a) ScPif1 and (b) Sgs1 binding to homopolymeric oligonucleotides. (c) ScPif1 and (d) Sgs1 binding to 20-mers comprised of 25% each dNTP (non-G-rich, NG) or 75% purines (G-rich, GR; Table S2). (e) ScPif1 and (f) Sgs1 binding to G4 structures. Error bars here and in all figures correspond to one standard deviation of the mean from ≥3 experiments.
