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. Author manuscript; available in PMC: 2013 Jun 13.
Published in final edited form as: Gastroenterology. 2011 Feb 13;140(5):1618–28.e16. doi: 10.1053/j.gastro.2011.02.009

Figure 4.

Figure 4

miR-517a and miR-520c promote cell proliferation, migration, and invasion in vitro. (A) Proliferation assay after transfection with control oligonucleotide, miR-517a, or miR-520c in Huh7 cells. Results are expressed as a percentage of 3H-thymidine incorporation (counts per million) ± standard error of the mean compared with control. (B) Transwell migration assay. Cells that migrated from the upper well of a Transwell chamber into the lower well were stained and counted. Magnification, ×100. (C) Cell invasion assays. Inserts coated with Matrigel were used to investigate the invasive potential of miR-517a and miR-520c transfected cells. Magnification, ×100. (D) Wound-healing assay. Cell monolayers were scratched and images were taken 0 and 24 hours after wounding. Results are expressed as distance (in μm) between the 2 edges ± standard error of the mean. Magnification, ×100.