Fig. 7.

ANG II infusion enhances NMDA receptor-mediated currents in spinally projecting PVN neurons. A: retrograde labeling of spinally projecting PVN neurons following microinjection of rhodamine-labeled microsphere into the thoracic spinal cord. Scale bar = 20 μm. B: whole cell patch-clamp recording on spinally labeled PVN neurons showing an enhancement of NMDA-induced currents in ANG II-infused mice (left and middle), an effect prevented by MnTBAP (100 μmol/l) (right). HP = −60 mV. C: enhancement of NMDA current density (pA/pF) in spinally labeled PVN neurons of ANG II-infused mice. This enhancement is suppressed by MnTBAP. **P < 0.01 vs. saline or MnTBAP, using ANOVA, and Tukey's test.