Fig. 5.

Effect of agouti-related peptide (AGRP) and SHU-9119 on food intake and hypothalamic gene expression. Mice received AGRP (0.1 nmol), SHU-9119 (1 nmol) or saline (control) via intracerebroventricular injection. A: after injection, food was provided and intake was measured from hours 0 to 2; then no food was provided from hours 2 to 4. B and C: hypothalamic Nenf and Bdnf gene expression was increased 4 h after initial injection, following access to food and increased food intake. Values are shown as means ± SE; *P < 0.05 vs. saline.