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qPCR primers used to measure hypothalamic gene expression
| Gene | Sense Primer | Antisense Primer |
|---|---|---|
| Nenf | 5′ GAAGGGAGTGGT GTTCGA 3′ | 5′ GTGTCGTGAGTGAGGTCTGC 3′ |
| Pomc | 5′ ATGCCGAGATTCTGCTACAG 3′ | 5′ TGCTGCTGTTCCTGGGGC 3′ |
| Agrp | 5′ ATGCTGACTGCAATGTTGCTG 3′ | 5′ CAGACTTAGACCTGGGA ACTCT 3′ |
| Bdnf | 5′ TCATACTTCGGTTGCATGAAGG 3′ | 5′ AGACCTCT CGAACCTGCCC 3′ |
| Mc4r | 5′ CGGACGGAGGATGCTATGAG 3′ | 5′ CGCCACGATCACTAGAATGTT 3′ |
| 18S rRna | 5′ GCAATTATTCCCCATGAACG 3′ | 5′ GGCC TCACTAAACCATCCAA 3′ |
