Effect of cortisol (500 ng/ml) on transepithelial resistance (TER; A), [3H]PEG-4000 flux (B), TRIC mRNA (C), and protein abundance (D), as well as immunolocalization of TRIC in control (Ct; E) and cortisol-treated (Co; F) cultured gill epithelia derived from rainbow trout. Data are expressed as mean values ± SE (n = 12–20). *P < 0.05, significant difference between Ct and Co epithelia. Scale bar = 25 μm in E and F.