Fig. 2.

Many cells, including GAD67+ neurons, received monosynaptic afferent input from the solitary tract (ST), and this innervation persisted into adulthood. A plot of latency vs. jitter (A) shows that most responses elicited by ST stimulation had a jitter value < 300 μs. A histogram of jitter values (B) shows the cutoff (dashed line) that we used to define mono- vs. polysynaptic innervation at 300 μs. Inset in B shows an example of raw data from a single neuron with an average latency of 1.69 ms and a jitter of 150 μs. GS, gabazine-strychnine.