Phosphorylation of mTOR at Ser2448 (A), S6K1 at Thr389 (B), and 4E-BP1 at Thr37/46 (C) during the initial and experimental basal post-absorptive periods during the Control (CON) and Rapamycin (RAP) trials. To control for potential day-to-day variations in basal protein metabolism, both the CON and RAP trials included an initial basal period. However, during the RAP trial, subjects were administered 16mg of rapamycin at the start of the experimental basal period, whereas no rapamycin was ingested during the CON trial. Data are mean±SEM, n=6.