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. 2013 May 21;108(11):2304–2311. doi: 10.1038/bjc.2013.243

Figure 2.

Figure 2

Detection of the genuine miRNA-663 and miRNA-744-binding sites in the 3′-UTR of eEF1A2. (A) Scheme representing the mutations of the mir-663 and mir-744 seed sequences in the eEF1A2 3′-UTR miRNA. Mutated regions are shown by rectangles. Names of mutations are in boldface type. (B) Mutations of the mir-663 and mir-744-binding sites increase eEF1A2 3′-UTR reporter activity. MCF7 cells were transfected with wild-type or mutant eEF1A2 reporter constructs, and luciferase activity measured 24 h later. Transfections of MCF7 cells with non-modified pSICheck-2 vector or wild-type eEF1A2 3′-UTR reporter were used as controls. Error bars represent s.d. values. n=3. *P<0.05 vs control. n=3. Student's t-test.