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. Author manuscript; available in PMC: 2013 Jun 13.
Published in final edited form as: J Cell Physiol. 2008 Nov;217(2):377–387. doi: 10.1002/jcp.21507

Fig. 2. Quantification of IGF-1R gene expression.

Fig. 2

VSMC from 4 pairs of young (6 mo) and old (24 mo) rats were grown in DMEM containing 5 mM (control) or 12.5 mM glucose for 3 days. A. Two representative RT-PCR gels show the 516 bp IGF-1R bands with 315 bp 18S-RNA control on the top; and the 424 bp of IGF-1 bands with the same 18S-RNA bands on the bottom. B. Expression of IGF-1R (n=4 per group) were quantified by custom-designed TaqMan®Gene Expression Assay on ABI Prism 7700. The individual p value is described in the text, and * indicates p ≤ 0.05 for comparisons between young (□) and old (■).