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. Author manuscript; available in PMC: 2013 Jun 13.
Published in final edited form as: J Cell Physiol. 2008 Nov;217(2):377–387. doi: 10.1002/jcp.21507

Fig. 3. Effect of IGF-1 on IGF-1R signaling.

Fig. 3

VSMC from young rats were grown in complete DMEM medium overnight and then changed to reduced medium with 5 nM IGF-1 for 0.5, 1, 2, 4, 6, 8 or 24 h. A. Images depicting the shuttling of P-Akt and P-FOXO3a between the nucleus and cytosol at the indicated times were captured using a confocal laser scanning microscope and shown in two panels: on the top, slides stained with anti-IGF-1R in green, anti-P-Akt in red; on the bottom, slides stained with anti-P-Akt in red and FOXO3a in green; both with DAPI-stained nuclei in purple. Each image includes three splits of single channel and one merged of three channels. B. On the left, an example of a Western blot shows cytoplasmic P-JNK vs. P-ERK after 0, 1, 2, 4, 6, 8 h of IGF-1 exposure with corresponding β-actin as a loading control. On the right, an example of a Western blot shows P-ERK1/2 vs. total ERK1/2 after 0, 0.5, 1, 2, and 3 h of IGF-1 exposure with the corresponding β-actin blot as a loading control.