Gapdh |
190 |
5′-CCTCTGGAAAGCTGTGGCGT-3′ |
5′-TTGGCAGGTTTCTCCAGGCG-3′ |
qRT-PCR |
PGC-1α |
166 |
5′-GAGCGCCGTGTGATTTACGT-3′ |
5′-GCGAAAGCGTCACAGGTGTA-3′ |
qRT-PCR |
FoxO6 |
172 |
5′-CTCACGCTCTCGCAGATCTA-3′ |
5′-GCATCCACCATGAACTCTTGC-3′ |
qRT-PCR |
PGC-1α |
252 |
5′-TTCGGCCTCAACTTCCTCATCTACCA-3′ |
5′-TATGACAGTGATCTGGGATGAGTCT-3′ |
CHIP assay (−99817 to -99566) |
PGC-1α |
402 |
5′-GCTTCATGGATGTGCTGGG-3′ |
5′-AGGTCATGGGCTCTACTTTC-3′ |
CHIP assay (−2390 to -1990) |
PGC-1α |
243 |
5′-GCAGAGGGCTGCCTTGGAGTG-3′ |
5′-ATCCAGCTCCCGAATGACGCCAGTC-3′ |
CHIP assay (−103 to +141) |
PGC-1α |
3251 |
5′-GTCCCTGCACATGGCTTAT-3′ |
5′-CAATCCACTCTGACACACA-3′ |
Promoter cloning (−3170 to +101) |
FoxO6 |
3276 |
5′-CCAGAGGAAGATAAGGATTAT-3′ |
5′-GATGAGGTCGGCGTAGGAAAG-3′ |
Promoter cloning (−2977 to +299) |
FoxO6 |
792 |
5′-CCAAGCTTGGGATGTGGGCGGCCA- |
5′-GGGATCCCGGCCCGGCTGCTGCGG- |
Cloning of FoxO6 (229-492)-His |
|
|
GCCCGGC-3′ |
CCGC-3′ |
|