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. 2013 Jun 13;33(3):e00045. doi: 10.1042/BSR20130031

Table 1. The sequences and amplicon sizes of the primer sets used in this study.

Gene Amplicon size (bp) Forward primer Reverse primer Purpose
Gapdh 190 5′-CCTCTGGAAAGCTGTGGCGT-3′ 5′-TTGGCAGGTTTCTCCAGGCG-3′ qRT-PCR
PGC-1α 166 5′-GAGCGCCGTGTGATTTACGT-3′ 5′-GCGAAAGCGTCACAGGTGTA-3′ qRT-PCR
FoxO6 172 5′-CTCACGCTCTCGCAGATCTA-3′ 5′-GCATCCACCATGAACTCTTGC-3′ qRT-PCR
PGC-1α 252 5′-TTCGGCCTCAACTTCCTCATCTACCA-3′ 5′-TATGACAGTGATCTGGGATGAGTCT-3′ CHIP assay (−99817 to -99566)
PGC-1α 402 5′-GCTTCATGGATGTGCTGGG-3′ 5′-AGGTCATGGGCTCTACTTTC-3′ CHIP assay (−2390 to -1990)
PGC-1α 243 5′-GCAGAGGGCTGCCTTGGAGTG-3′ 5′-ATCCAGCTCCCGAATGACGCCAGTC-3′ CHIP assay (−103 to +141)
PGC-1α 3251 5′-GTCCCTGCACATGGCTTAT-3′ 5′-CAATCCACTCTGACACACA-3′ Promoter cloning (−3170 to +101)
FoxO6 3276 5′-CCAGAGGAAGATAAGGATTAT-3′ 5′-GATGAGGTCGGCGTAGGAAAG-3′ Promoter cloning (−2977 to +299)
FoxO6 792 5′-CCAAGCTTGGGATGTGGGCGGCCA- 5′-GGGATCCCGGCCCGGCTGCTGCGG- Cloning of FoxO6 (229-492)-His
GCCCGGC-3′ CCGC-3′