Fig. 6.

Glial inclusions. Representative double immunofluorescence images probed with α-synuclein (red) and the oligodendroglial marker, olig2 (green). Composite merged images show α-synuclein immunoreactive inclusions in oligodendrocytes resembling GCIs in the white matter of the frontal cortex (a, d), the pons (b) and the alveus of the hippocampus (c). Close proximity is observed between a subset of activated microglia, as detected by iba-1 (green) and α-synuclein (red) inclusion containing neurons, this occasionally involved encircling of α-synuclein-containing neurons by iba-1-positive processes (e–g). A small proportion of these microglia contained α-synuclein immunoreactivity (g, inset arrowheads). A high level of reactive astrogliosis was detected throughout all brain regions examined, without evidence of α-synuclein expression within astrocytes or their processes (h–j). DAPI nuclear stain (blue). Scale bar in j represents 50 μm (a–j). Scale bar in g inset represents 25 μm