Phosphorylation of p38 MAPK in CSF prior to photothrombosis (PTI) (0 min) and as a function of time (hour) after PTI in vehicle, or treated with tPA (2 mg/kg iv), RBC-tPA (0.1 mg/Kg iv), U 0126, SB 203580, SP 600125 or D-JNKI1 (all 1 mg/kg iv), n=5. Data expressed as percent of control by ELISA determination of phospho MAPK and total MAPK isoforms and subsequent normalization to total form. A: pretreatment 30 min before H/I B: post treatment 2h after H/I. *p<0.05 versus corresponding 0 time value +p<0.05 versus corresponding PTI non treated value #p<0.05 versus corresponding PTI + tPA value.