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. Author manuscript; available in PMC: 2014 Apr 25.
Published in final edited form as: Cell Rep. 2013 Apr 11;3(4):1140–1152. doi: 10.1016/j.celrep.2013.03.025

Figure 3. C-kit and Sox10 expression can be used to identify and isolate melanoblasts.

Figure 3

(A) Melanocyte progenitors can be identified in day 11 NC protocol-derived populations by coexpression of Sox10::GFP and the melanocyte transcription factor MITF (arrowheads). Scale bar represents 50µm. (B) Flow cytometry reveals the presence of a Sox10::GFP and c-kit co-expressing population. C-kit “fluorescence minus one” (FMO) was used a negative control for c-kit staining. (C) 4-way FACS sorting for Sox10::GFP and c-kit reveals an enrichment of the melanocyte markers MITFM and Dct in the SOX10/ckit double positive population by qRT-PCR. All error bars represent the s.e.m of at least three independent experiments. See also Figure S4.