Fig. 3.

(A) Genotoxic effect of acute THS on human genomic DNA as measured by the Comet assay. HepG2 cells were exposed to undiluted THS or SHS samples at 37^°C for 24h. The extent of DNA damage was analysed by measuring the % DNA in tail to total DNA from 90 randomly selected cells. The histogram depicts the average median % of DNA in tail ± SEM of three independent experiments. (B) HepG2 cells were exposed to chronic THS at varying dilutions under identical conditions as above.