Fig. 5.

Induction of oxidative DNA damage in human lung epithelial cells after 24h exposure to chronic THS. Cultured BEAS-2B cells were treated with three concentrations of THS extracts for 24h followed by LA–QPCR analysis. Representative gels show PCR amplified fragments of HPRT and POLB genes. Amplification of the large fragment was normalised to the amplification product of a small fragment of the corresponding gene. Quantitation of the amplified products is represented in the histograms with relative amplification arbitrarily set as 100. Each experiment was repeated three times.