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. 2013 Mar 5;28(4):381–391. doi: 10.1093/mutage/get013

Table II.

Concentrations of THS constituents in DMEM media (ng/ml) and in cellulose substrates (ng/g)

Sample name COT N-Formylnornicotine NNN NNK NNA Nicotelline 2,3′-Bipyridine PON
Acute THS
DMEM only BLOQ BLOQ BLOQ BLOQ BLOQ BLOQ BLOQ BLOQ
Blank BLOQ BLOQ BLOQ BLOQ BLOQ BLOQ BLOQ BLOQ
THS DMEM 27.0 50.9 0.264 0.903 0.43 0.752 189 228
3MM paper n.a. n.a. BLOQa 15.9a 1.6a n.a. n.a. n.a.
THS + HONO DMEM 14.9 27.0 0.145 0.517 1.82 0.312 107 163
3MM paper n.a. n.a. BLOQa 18.1a 5.4a n.a. n.a n.a.
SHS 11.3 23.6 0.259 0.149 BLOQ 0.404 90.2 134
Chronic THS
DMEM only BLOQ BLOQ BLOQ BLOQ BLOQ BLOQ BLOQ BLOQ
Blank BLOQ BLOQ BLOQ BLOQ BLOQ BLOQ BLOQ BLOQ
THS DMEM 133 585 1.57 7.20 0.39 8.04 147 13.0
3MM paper n.a. n.a. 19.5a 78.6a 1.4a n.a. n.a. n.a.

BLOQ = below the limit of quantitation.

aTSNA concentrations were obtained using GC–IT–MS/MS method and are expressed in nanogram per gram of 3MM paper (ng/g).