TABLE 1.
Control2 | Biofortified3 | |
Expt. 1 | ||
Total Se, μmol/L | 0.5 ± 0.2 | 5.4 ± 0.6* |
NBS, μmol/L | 0.02 ± 0.01 | 1.5 ± 0.1* |
PBS, μmol/L | 0.48 ± 0.1 | 4.2 ± 0.6* |
NBS, % | 4.0 ± 2.8 | 24.6 ± 1.3* |
PBS, % | 96.0 ± 2.8 | 75.4 ± 5.9* |
Protein, g/L | 35.5 ± 3.3 | 36.1 ± 3.4 |
GPX3, units/mL | 8.1 ± 2.8 | 28.8 ± 4.5* |
LDH, units/mL | 184 ± 8.9 | 102 ± 10.8* |
Expt. 2 | ||
Total Se, μmol/L | 2.6 ± 0.2 | 6.2 ± 0.8* |
GPX3, units/mL | 37.9 ± 0.9 | 35.6 ± 1.6 |
Sepp1,4 % | 100 ± 7.2 | 157 ± 23.1* |
Values are means ± SEs, n = 3–6. Means with * are different from the controls, P < 0.05. GPX3, plasma glutathione peroxidase; LDH, lactate dehydrogenase; NBS, nonprotein-bound Se; PBS, protein-bound Se; Sepp1, selenoprotein P.
In Expt. 1, the control diet contained 0.02 mg Se/kg; in Expt. 2, the control diet was supplemented with 0.3 mg Se/kg.
In Expt. 1, the biofortified diet was supplemented with 3.0 mg Se/kg as sodium selenite; in Expt. 2, the biofortified diet was supplemented with 1.0 mg Se/kg as Se-enriched yeast.
Values are relative density from the protein band.