Table 2.
Near Infrared calibration models and validation for glycerol and biomass in Pichia pastoris chemostat cultures
|
Model |
Range (g/L) |
λ (nm) |
LV |
Calibration |
Cross-validation |
External validation |
RMSEP RMSECV |
|||
|---|---|---|---|---|---|---|---|---|---|---|
| RMSEC (g/L) | R2 | RMSECV (g/L) | R2 | RMSEP (g/L) | R2 | |||||
|
Glycerol |
0-40 |
1500-1800 |
3 |
1.70 |
0.98 |
3.38 |
0.95 |
2.90 |
0.95 |
0.86 |
| Biomass | 0-30 | 1250-1350 | 5 | 0.48 | 0.97 | 0.93 | 0.91 | 1.17 | 0.94 | 1.26 |
λ: wavelength; LV: number of latent variables; RMSE: root-mean square error; R2: correlation coefficient.