Table 5.
Primers used in this study
| Primer | Sequence* | Purpose |
|---|---|---|
| sacBF |
cggcgactagttgagctgttgacaattaatcatcgtgtggtaccatgtgtggaattgtgagcggataacaattccgcgggttctttaggcccgtagtct (SpeI,SacII) |
PCR for the sacB gene |
| sacBR |
gccgcgatatctctcgtgatggcaggtt (EcoRV) |
PCR for the sacB gene |
| pk18msF |
gcgccgatatcgttcgtctggaaggcagta (EcoRV) |
PCR for the backbone of pK18mobsacB except for the sacB gene |
| pk18msR |
gcgcgactagtgcatgggcataaagttgc (SpeI) |
PCR for the backbone of pK18mobsacB except for the sacB gene |
| argR-F5 |
cgctggatcctttaagcacggcgttattt (BamHI) |
PCR for the upstream fragment of the argR gene |
| argR-R5 |
cggtctagatgcgagtcacgggattta (XbaI) |
PCR for the upstream fragment of the argR gene |
| argR-F3 |
cggtctagaggtaaggtataacccgagtgt (XbaI) |
PCR for the downstream fragment of the argR gene |
| argR-R3 |
cgatgtcgacgacttgatgcccacgaga (SalI) |
PCR for the downstream fragment of the argR gene |
| GargBF |
cgctctagaaaggacacaggcatgaatgact (XbaI) |
PCR for the argB gene from C. glutamicum |
| GargBR |
cgggtcgacttacagttccccatcctt (SalI) |
PCR for the argB gene from C. glutamicum |
| EargBF |
cgttctagaaggaggggtgcaatgatgaat (XbaI) |
PCR for the argB gene from E. coli |
| EargBR |
gcggtcgaccttaagctaaaatccg (SalI) |
PCR for the argB gene from E. coli |
| zwf-F |
ccgcctctagaaaggagaccatcatgagcacaaacac (XbaI) |
PCR for the zwf gene from C. glutamicum |
| zwf-R |
cggtagtcgacccctaaattatggcctgc (SalI) |
PCR for the zwf gene from C. glutamicum |
| ppnK-F |
gccatgaattcaaggacgcaataatgactgcacccacgaa (EcoRI) |
PCR for the ppnK gene from C. glutamicum |
| ppnK-R |
ccgccgagctccgaattaccccgctgac (SacI) |
PCR for the ppnK gene from C. glutamicum |
| gnd-F |
gcgatggtaccaaggagaccactatgccgtcaagtacgat(KpnI) |
PCR for the gnd gene from C. glutamicum |
| gnd-R |
ccgcgtctagaaaaggagagcctttaagct (XbaI) |
PCR for the gnd gene from C. glutamicum |
| pntAB-F |
cagggtacctcatcaataaaaccg(KpnI) |
PCR for the pntAB gene from E. coli |
| pntAB-R |
cgtctgcagttacagagctttcag(PstI) |
PCR for the pntAB gene from E. coli |
| qpgiF |
cccttctattctcggtgc |
qRT-PCR for pgi |
| qpgiR |
aggtcatttgcctgctgt |
qRT-PCR for pgi |
| qpfkAF |
tatccctgttgtcggtgtc |
qRT-PCR for pfkA |
| qpfkAR |
gtgagattcagcggtggt |
qRT-PCR for pfkA |
| qgapF |
ggaagttgaatacgacgatga |
qRT-PCR for gap |
| qgapR |
gcccagtccaggttcttt |
qRT-PCR for gap |
| qpycF |
accgccacgaaatccc |
qRT-PCR for pyc |
| qpycR |
aacggctgcgtagttgtct |
qRT-PCR for pyc |
| qpykF |
ccgtgcagtcggtattct |
qRT-PCR for pyk |
| qpykR |
gcgttccctctacatcgt |
qRT-PCR for pyk |
| qgltAF |
cgggaatcctgcgttac |
qRT-PCR for gltA |
| qgltAR |
tggcgaatctcgtcgtt |
qRT-PCR for gltA |
| qgdhF |
ccgccacatcggtgagta |
qRT-PCR for gdh |
| qgdhR |
agccatgcgacggtagt |
qRT-PCR for gdh |
| qargBF |
ggtttggtcggagacatca |
qRT-PCR for argB |
| qargBR |
gcctggagcaatcgtagag |
qRT-PCR for argB |
| qargJF |
cctgacatggcgttgg |
qRT-PCR for argJ |
| qargJR |
ctcggctcaccttcaca |
qRT-PCR for argJ |
| qzwfF |
acccgcaggataaacga |
qRT-PCR for zwf |
| qzwfR |
gctagatcataaatggc |
qRT-PCR for zwf |
| qppnkF |
gtttaccgaccgacttgtg |
qRT-PCR for ppnk |
| qppnkR |
gctgacctgggatctttatt |
qRT-PCR for ppnk |
| qicdF |
aggaccagggctacgacat |
qRT-PCR for icd |
| qicdR |
gcggaacccttaacagc |
qRT-PCR for icd |
| qgndF |
aaccgcagcactgacaaa |
qRT-PCR for gnd |
| qgndR |
cagggatgctacgaactct |
qRT-PCR for gnd |
| 16s-F |
tcgatgcaacgcgaagaac |
qRT-PCR for 16srRNA |
| 16s-R | gaaccgaccacaagggaaaac | qRT-PCR for 16srRNA |
*Restriction enzyme sites are underlined.