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. 2013 Jun 13;9(6):e1003562. doi: 10.1371/journal.pgen.1003562

Figure 3. Augmin accumulation at spindle poles is correlated with chromosome congression.

Figure 3

(A, B) GFP-Dgt2 and Wac-GFP localise to wild-type acentrosomal spindle poles. (C) Dgt6 localises to spindle poles in wild-type oocytes by immunostaining. (D) The Augmin level in spindle pole regions is well correlated with the level of chromosome congression. Live oocytes expressing GFP-Dgt2 and Rcc1-mCherry were used to measure two parameters: the spread of the chromosome mass (including the 4th chromosomes) along the spindle axis (as in Figure 1B), and the intensity of GFP-Dgt2 signal above the background (as in Methods & Materials) for each spindle. Correlation between the chromosome spread and the log of GFP-Dgt2 intensity is significant (r = −0.772, p<0.01, n = 26).