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. Author manuscript; available in PMC: 2013 Jun 13.
Published in final edited form as: Nat Chem Biol. 2010 Oct 3;6(11):829–836. doi: 10.1038/nchembio.453

Figure 2. Pyrvinium inhibits Wnt signaling in vivo.

Figure 2

(a–c) Pyrvinium blocks secondary axis induction in Xenopus in a dose-dependent manner. Embryos (four- to eight-cell stage) were injected ventrally with Xwnt8 mRNA (0.5 pg) plus vehicle (a) or pyrvinium pamoate (200 μM) (b), allowed to develop and scored for secondary axis formation (c). n = number of embryos. (d, e) Pyrvinium blocks expression of chordin. Embryos injected with Xwnt8 plus vehicle (d) or pyrvinium pamoate (200 μM) (e) were probed by in situ hybridization (stage 10.5) for chordin (arrowheads). Vegetal view, dorsal side up. Scale bars, 800 μm for a, b and 400 μm for d, e. (f) Pyrvinium inhibits Xwnt8 induction of Wnt target genes Siamois and Xnr3 in Xenopus animal cap explants. RT-PCR of total RNA extracted from animal caps. WE, whole embryo; RT, reverse transcriptase; ODC, ornithine decarboxylase, loading control.