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. 2013 Jun 6;6:167. doi: 10.1186/1756-3305-6-167

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Copyright © 2013 Fneich et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://www.creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Methylation sensitive restriction assay. Genomic DNA of B. glabrata: Brazilian strain (B. glabrata Bre) and Guadeloupian strain (B. glabrata Gua), of positive controls: O. mykiss and HeLa cells and of negative controls: S. mansoni (miracidia) was digested with the MspI enzyme, its methylation sensitive isoschizomer HpaII or was not digested (ND). 200 ng of DNA were loaded per well. Digestion profile was observed on an ethidium bromide stained 1% agarose gel. Lad: 1 kb DNA ladder (promega), lowest band 1500 bp, highest band 10000 bp.