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. 2013 Jun 13;9(6):e1003531. doi: 10.1371/journal.pgen.1003531

Figure 4. PARP-1 or vimentin is sufficient to reverse EMT and confer increased cell motility.

Figure 4

(A) Melanoma (G361) and endothelial (HUVEC) (B) cells were silenced for PARP-1 or vimentin and the expression levels of Axl, E-/VE-cadherin, Snail1, ILK, β-catenin, GSK-3β, PARP-1, and vimentin were determined by immunoblot. (C) HUVEC were silenced for vimentin and wound healing was measured. After over-expression of vimentin wound healing closure was measured in HUVEC cells (D) or B16-F10 (E). (F) Cell migration was analyzed in epithelial cell line Madin Darby canine kidney (MDCK) cells transfected with either GFP or GFP-vimentin using video-microscopy and MetaMorph Image Analysis software. While vimentin was able to increase the length of the trajectories in the absence or presence of hepatocyte growth factor (HGF), treatment with PARP inhibitor resulted in a sustained reduction in cell motility (*P<0.05 PJ-34 or olaparib versus control).