FIGURE 3:

(A) HEK-293T cells were transfected with either 100 pmol of scrambled siRNA or Mib1 siRNA for 72 h. Mib1 and SMN protein levels were determined after 72 h by Western blotting. (B) HEK-293T cells were transfected with either 100 μM negative control miRNA or miR-137 for 72 h. SMN protein levels were determined by Western blotting. The data represent the mean ± SEM of three independent experiments. (C) SMA patient–derived fibroblasts (3813) were transfected with Mib1 siRNA or scrambled control. Cells were harvested 72 h post-transfection, and Mib-1 mRNA and SMN protein levels were assessed by RT-PCR and Western blot analysis, respectively. (D) HEK-293T cells were transfected with Mib1 siRNA, miR-137, or scrambled control. Cells were harvested 72 h posttransfection, and SMN mRNA level was analyzed by RT-PCR. *, p < 0.05; **, p < 0.01; ***, p < 0.001.