FIGURE 5:

(A) HEK-293T cells were transfected with 2 μg full-length SMN or SMNΔ7 and 1 μg Mib1-FLAG and HA-Ub cDNAs. The cells were harvested 48 h later, SMN was immunoprecipitated and resolved by SDS–PAGE, and the proteins were analyzed by Western blotting. The blots were probed with an HA antibody to detect ubiquitinated SMN. (B) Pulse–chase analysis of full-length SMN and SMNΔ7, in the absence or presence of 2 μg Mib1-myc. The data represent the mean ± SEM of three experiments.