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. 2013 Jun 15;24(12):1904–1917. doi: 10.1091/mbc.E12-10-0775

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© 2013 Tebbs and Pollard. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Only the Ras-GAP–related C-terminal domain of Rng2p is essential for viability. Complementation experiments with rng-D5 or rng2Δ cells expressing full-length mEGFP-Rng2p or mEGFP-Rng2p deletion constructs from the leu1 locus under the control of the rng2 promoter. Photographs of agar plates with spots containing serial 1:10 dilutions of cells incubated for 48 h at the permissive (25°C) or restrictive (36°C) temperature.