Table 1.
List of primers utilised in the HRM analysis
| Amplicon | Forward primer | Reverse primer | Product length [bp] | Annealing temperature [°C] |
|---|---|---|---|---|
| 1a |
GGAAGTCGGAACACAAGGAA |
CATCAGGTACTTGCCGATGA |
231 |
60 |
| 1b |
CTCCACCGAGGTCATCTACC |
CCCCAGCAAGCCATACTTAC |
221 |
60 |
| 2 |
AGGCCATCATCCTGACGTT |
CCCAACACCGGAAAGGATA |
159 |
60 |
| 3 |
CTCCCTGGGCCTGTGAGT |
CCCTGCCCCGCGCACGCA |
165 |
60 |
| 4a |
CAGGACGGGTGTGTGCTG |
CCCTAGCACGTGCCTACCT |
175 |
60 |
| 4b |
GCCTGGAGTACCTGCATAGC |
GTGCAGCCCTCAGGGAGT |
172 |
60 |
| 5a |
CACTCCCTGAGGGCTGCAC |
GGGGCACTTACAGGGTGAC |
180 |
60 |
| 5b |
GGCTTCAAGGTGGACATCTG |
CTGTGGCCAGAGAGGGTCT |
161 |
60 |
| 6 |
TCAACCACCTTGACTGACCA |
ACCCCCAACCCTACATTTCT |
249 |
60 |
| 7 |
CCTTAGGAGCGTCCAGGTATC |
CTCAACCAGCTGCCCACAT |
244 |
60 |
| 8a |
CTGGGTCGGAAAACTGGAC |
GTGCAGGTCCTCCAAGTACG |
182 |
60 |
| 8b |
GAGCCCAGACACCAAGGAC |
GACATCCTGGCCGAGTCA |
188 |
60 |
| 9 | TCAGCTCAGGCCACACTTGC | AGCCTCACTGCTGCTTGC | 232 | 60 |